HCV Genotype Diagnostic Kit (Fluorescence PCR)

【Principle】

This test kit applies magnetic bead method to extract viral nucleic acid from Hepatitis C virus (HCV) positive serum or plasma specimens. It uses HCV 1b / 2a/ 3a / 3b / 6a specific primers and fluorescent probes, matched with RT-PCR reaction solution to realize the rapid genotyping of HCV 1b / 2a/ 3a / 3b / 6a specific RNA nucleic acid fragments through Real-time PCR detection and high efficiency internal standard technologies.

【Intended use】

A large number of clinical and basic studies have found that people infected with different genotypes of Hepatitis C virus (HCV) have differences in clinical manifestations, hepatitis severity, antiviral diagnosis and treatment. This test kit is used for in vitro qualitative detection of hepatitis C virus 1b, 2a, 3a, 3b and 6a genotypes in serum or plasma specimens, so as to help medical professionals understand the Hepatitis C virus infection of patients and determine appropriate treatment methods.

【Characteristics】

【Advantages】

● Magnetic bead extraction of nucleic acid has the advantages of simple and rapid operation and high nucleic acid extraction efficiency;

● The specific RT-PCR amplification reaction system has strong anti-interference ability, and there is no crossover among HCV genotypes;

● High sensitivity and precision, both inter-assay and intra-assay coefficient of variation (CV)  ≤ 5%;

● High efficiency internal standard technology to avoid false negative test results;

● 2a/6a mixed system and 3a/3b mixed system are adopted to reduce the number of extraction and realize accurate genotyping detection.