Human Immunodeficiency virus type I Diagnostic Kit(Fluorescence PCR)

【Principle】

This test kit applies magnetic bead method to extract viral nucleic acid from serum or plasma specimens.It uses a pair of specific primers and a specific fluorescent probe designed for the conserved region of human immunodeficiency virus type I (HIV-1) nucleic acid RNA, matching with RT-PCR reaction solution to realize the quantitative detection of HIV-1 RNA through Real-time PCR detection and high efficiency internal standard technologies.

【Intended use】

This test kit is used for direct quantitative detection of human immunodeficiency virus (HIV-1) nucleic acid RNA in clinical serum or plasma specimens. It can effectively improve the accuracy and precision of HIV-1 infection detection. The test results can be used for the auxiliary diagnosis of HIV-1 infection and the clinical effect monitoring of anti-HIV-1 drug treatment. It can dynamically reflect the changes of HIV-1 RNA titer during treatment and provide indicators for evaluating the curative effect.

【Characteristics】

【Advantages】

● Extraction of nucleic acid by magnetic bead method, simple operation and high extraction efficiency;

● The specific RT-PCR amplification reaction system has strong anti-interference ability. Test results of serum specimens with hemolysis, hyperbilirubin, hyperlipidemia and common antiviral drugs are not affected;

● High analytical sensitivity, accurate quantification and good repeatability, both inter-assay and intra-assay coefficient of variation (CV)  ≤ 5%;

● High efficiency internal standard technology to avoid false negative test results.